Fig. 3: Hepatocyte NAMPT protects against diet-induced glucose intolerance.
A Body weights over time in WT and NAMPTLKO mice on 12wk Western diet. n = 8 WT, 6 NAMPTLKO mice. B WT and NAMPTLKO mice were subjected to 12wk Western dietary feeding and metabolic phenotyping by indirect calorimetry at 4wk and 12wk post-initiation of dietary intervention. Mean heat generation during light and dark cycles is demonstrated for light and dark cycles. n = 5 WT, 4 NAMPTLKO mice. C Fat and lean mass and % body fat in WD-fed WT and NAMPTLKO mice. n = 8 WT, 6 NAMPTLKO mice. D Circulating lipids in 12wk WD-fed WT and NAMPTLKO mice. n = 8 WT, 6 NAMPTLKO mice. TG, triglycerides; LDL-C, low-density lipoprotein cholesterol. E Serum transaminases, albumin and liver weight-to-body weight ratios in 12wk WD-fed WT and NAMPTLKO mice. n = 8 WT, 6 NAMPTLKO mice. F 4 h fasting insulin and glucose in 12wk WD-fed WT and NAMPTLKO mice. n = 8 WT, 6 NAMPTLKO mice. G and H Insulin tolerance and glucose tolerance testing in WT and LKO mice after 12wk WD feeding. n = 8 WT, 6 NAMPTLKO mice. *P < 0.05 vs. NAMPT WT. Error bars in (A–F and H) represent SEM. Circles, Western diet-fed WT, Squares, Western diet-fed NAMPTLKO. Statistical tests: (A, G, H), repeated measures ANOVA. B–F Two-tailed T-test.
