Fig. 7: Hepatocyte SIRT1 selectively modulates anti-lipogenic effects of hepatocyte NAMPT overexpression.

A Serum TG, cholesterol and LDL in Western diet-fed WT and SIRT1^LKO mice overexpressing empty vector or NAMPT. TG, triglycerides. LDL-C, low-density lipoprotein cholesterol. Interactions, P = ns for all circulating lipid analyses. n = 4 SIRT1^fl/fl Chow; 11 SIRT1^fl/fl EV WD; 6 SIRT1^fl/fl AAV8-NAMPT WD; 4 SIRT1^LKO EV WD; 6 SIRT1^LKO AAV8-NAMPT WD. B Liver weight-to-body weight ratios in mice analyzed in (A). Interaction P = ns. n = 3 SIRT1^fl/fl Chow; 9 SIRT1^fl/fl EV WD; 6 SIRT1^fl/fl AAV8-NAMPT WD; 5 SIRT1^LKO EV WD; 5 SIRT1^LKO AAV8-NAMPT WD. C De novo lipogenic gene expression in mice analyzed in (B). Linear substrate pathway is shown at left for illustrative purposes. Interaction P = ns for LPK, ACC1, FASN, ELOVL6. Interaction P < 0.05 and <0.001 for GPAT and SCD1. *, **, ***, P < 0.05, <0.01, <0.001 vs. bracketed control by Tukey’s multiple comparisons testing. n = 4 SIRT1^fl/fl Chow; 11 SIRT1^fl/fl EV WD; 6 SIRT1^fl/fl AAV8-NAMPT WD; 4 SIRT1^LKO EV WD; 6 SIRT1^LKO AAV8-NAMPT WD. Error bars in (A–C) represent SEM. Circles, SIRT1^fl/fl Chow; Squares, SIRT1^fl/fl EV WD; Upward Triangles SIRT1^fl/fl AAV8-NAMPT WD; Downward Triangles, SIRT1^LKO EV WD; Diamonds SIRT1^LKO AAV8-NAMPT WD. Statistical tests: (A–C), two-way ANOVA; C Tukey’s post hoc correction.