Fig. 1: IgG glycoengineering by ENGases.
From: Mechanism of cooperative N-glycan processing by the multi-modular endoglycosidase EndoE
ENGases can be used to remodel the glycosylation pattern of IgG antibodies. In the first step, an ENGase is used to cleave the variety of glycoforms present in the IgG antibody. In the second step, additional enzymes such as α-fucosidase can be used to remove specific glycan moieties that are not cleaved by the ENGase. In the third step, a glycan-oxazoline donor is used along with an ENGase glycosynthase mutant in order to transfer a specific glycan and obtain a homogeneously N-glycosylated IgG antibody.
