Fig. 10: EndoE model of action.
From: Mechanism of cooperative N-glycan processing by the multi-modular endoglycosidase EndoE
a Schematic representation of CT N-glycan processing by EndoE. In a first step, EndoE-GH20 cleaves the non-reducing terminal GlcNAc residues from the CT N-glycan substrate, generating smaller glycoforms that can be processed by EndoE-GH18. In a later stage, EndoE-GH18 cleaves the chitobiose core of the N-linked glycan, releasing the glycan from the protein substrate. b Schematic representation of HM-type N-glycan processing by EndoE. As EndoE-GH20 has no activity on mannose, EndoE-GH18 is the only domain acting on HM-type N-glycan substrates, directly cleaving the chitobiose core of the N-linked glycan and releasing the glycan from the protein substrate.
