Fig. 3: Tig1 overexpression disrupts regeneration of distal elements.
From: Tig1 regulates proximo-distal identity during salamander limb regeneration
a Schematic of the experimental approach. b Tig1 Bv transduction from mid bud blastema onwards leads to shortening of the regenerated limb through malformations in the distal limb elements, as analysed through Alcian blue/Alizarin red staining (n = 15 biologically independent animals). c Quantification of limb length. The box horizontal boundaries define the interquartile range (upper boundary: 75th percentile and bottom boundary: 25th percentile of dataset values), the internal line indicates Median, whiskers indicate highest and lowest value for each condition. **p = 0.0014 (two-tailed paired-matched t-test). d Kernel density estimation of lengths for the indicated limb segment. The coloured diamonds on the x axis represent individual measurements. **p = 0.0014; ***p = 0.0004 (two-tailed paired-matched t-test). A broadening of the distribution towards shorter lengths is observed upon Tig1 overexpression for whole limb, lower arm and hand, while no changes are observed in the upper arm. Source data are provided as a Source Data file.
