Fig. 4: Tig1 induces distal-to-proximal displacement of blastema cells.
From: Tig1 regulates proximo-distal identity during salamander limb regeneration
a Representative images of the displacement assay. Cells in the distal third of a 4-day blastema give rise to distal elements9. Upon electroporation of a control vector (n = 7) to enable tracing during regeneration, they contribute primarily to digits (first column). Overexpression of Prod1 (n = 8), Tig1 or Tig1P155A (n = 13) coupled to fluorescent proteins promotes autologous displacement of cells from distal to proximal positions during limb regeneration. dpe days post electroporation. Scale bar: 1 mm. b Quantification of the percentage of animals with electroporated cells found in the indicated limb segments. c An image analysis algorithm for the automatised quantification of cells (by fluorescence intensity) along the PD axis. f indicates the number of pixels whose intensity is higher than the threshold; f/fmax indicates the ratio between each f and its maximal value along the anatomical axis. d Distribution of fluorescence intensity along the PD axis for the indicated conditions. Coloured shading indicates standard deviation, coloured centre indicates mean. Grey shading indicates the standard deviation of the PD position corresponding to elbow (E) or wrist (W). An amputation plane. Source data are provided as a Source Data file.
