Fig. 5: Tig1 mediates proximo-distal cell-surface interactions.
From: Tig1 regulates proximo-distal identity during salamander limb regeneration
a Schematic of the engulfment assay. Juxtaposition of proximal (P) and distal (D) blastemas results in the engulfment of the distal one by its proximal counterpart. b Representative images of juxtaposed proximal (red) and distal (white) blastemas at the indicated times post juxtaposition, and after the indicated treatments. Note lack of engulfment following treatment with anti-TIG1 (α-TIG1) antibodies raised against two extracellular epitopes. Scale bar: 500 µm. c Quantification of engulfment for the indicated pairs (pooled data from three independent experiments). For α-Prod1, α-Tig1 and α-TMEFF1, [antibody] = 20 µg/ml. d Images along an X-stack (x1 to x5) of representative PD blastema pairs at 5 days post juxtaposition (5dpj), following treatment with the indicated antibodies. Proximal blastemas derive from caggs:eGFPEtnka axolotls. Scale bar: 200 µm. e Quantification of the overlap between proximal and distal blastemas at 5dpj, based on (d) (n = 5 biologically independent samples). ***p = 0.0012 (two-tailed unpaired t-test). Error bars represent SEM. Source data are provided as a Source Data file.
