Fig. 8: Genes up and downregulated by Tig1 map on opposite ends of the proximo-distal axis of the regenerating limb.
From: Tig1 regulates proximo-distal identity during salamander limb regeneration
a Contribution of up- and downregulated genes by Tig1 overexpression to PC1 (see Fig. 1b). Note that most genes upregulated by Tig1 overexpression map to the proximal portion of PC1, whereas genes downregulated by Tig1 overexpression contribute to the distal portion. b Up- and downregulated genes by Tig1 overexpression were used to calculate either an upregulation (right) or a downregulation (left) score for each cell as well. c Boxplots represent the gene expression levels for the given genes among the top 100 cells expressing Tig1-upregulated genes (red) and top 100 expressing Tig1-downregulated genes (blue) cells based on the scoring in (b). The box horizontal boundaries define the interquartile range (upper boundary: 75th percentile and bottom boundary: 25th percentile of dataset values), the internal line indicates Median, whiskers indicate highest and lowest value for each condition excluding outliers. Results of a t-test comparing the gene expression distributions for the two groups are shown above each boxplot panel.
