Fig. 1: Demyelination of the nonischemic CC after dMCAO.

A Electric cauterization (EC) was conducted at the left distal MCA just before the bifurcation. B, C Representative CBF images and quantitative analysis before, during and 24 h after surgery. (n = 6 mice; mean ± S.D.; ***P < 0.001 vs. baseline; one-way ANOVA, Tukey post hoc test). D Representative images of serial coronal slices in T2 and DWI sequences. E Quantification of infarct volume according to DWI sequence (n = 6 mice; mean ± S.D.; ***P < 0.001 vs. sham; paired t-test). F Representative Nissl staining showing ischemic lesion (red dotted frame) and the location of nonischemic CC for analyses (red asterisk). G, H Representative MBP, MAG and NF200 immunostaining in the ipsilateral CC and their relative fluorescence intensity ratios. Data were normalized to the signal intensity of sham mice (n = 5 mice; mean ± S.D.; *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham; paired t-test). Scale bar, 50 μm. I, J Immunoblotting analyses of MBP and MAG in sham and dMCAO mice. Protein β-actin served as control (n = 5 mice; mean ± S.D.; **P < 0.01 vs. sham; paired t-test). Protein samples derived from the same experiment and gels/blots were processed in parallel. K Representative images of black gold and LFB staining. Scale bar, 50 μm. L Quantitative analysis of myelinated area according to black gold staining (n = 5 mice; mean ± S.D.; ***P < 0.001 vs. sham; paired t-test). M Quantitative analysis of white matter damage severity according to LFB staining (n = 5 mice; mean ± S.D.; ***P < 0.001 vs. sham; paired t-test). N–R Representative EM images and statistical analyses of myelin thickness, myelinated axons and g-ratio in sham and dMCAO groups (n = 5 mice; mean ± S.D.; **P < 0.01, ***P < 0.001 vs. sham; paired t-test). Scale bar, 2 μm. Source data are provided as a Source Data file.