Fig. 10: Lrp1 knockdown reverses LCN2-induced demyelination following cortical infarction.
From: Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice
A, B Representative MBP, MAG and NF200 staining and statistical analyses of the CC in dMCAO mice injected with LC-NC or LV-Lrp1-RNAi (n = 5 mice; mean ± S.D.; **P < 0.01 vs. LC-NC; paired t-test). Scale bar, 50 μm. C, D Immunoblotting analyses showing the changes of MBP and MAG expressions after transfection (n = 5 mice; mean ± S.D.; *P < 0.05, **P < 0.01 vs. LC-NC; paired t-test). Protein samples derived from the same experiment and gels/blots were processed in parallel. E, F Representative black gold staining and quantification of dMCAO mice receiving lentivirus (n = 5 mice; mean ± S.D.; **P < 0.01 vs. LC-NC; paired t-test). Scale bar, 50 μm. G–K Representative EM images and quantifications from two groups (n = 5 mice; mean ± S.D.; **P < 0.01, ***P < 0.001 vs. LC-NC; paired t-test). Scale bar, 2 μm. Source data are provided as a Source Data file.
