Fig. 5: Absence of Lcn2 attenuates astrocytic phagocytosis in vitro.
From: Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice
A Representative immunostaining images of astrocytic phagocytosis, GFAP-positive (gray), lysosomes-positive (LysoTracker Red dye, red) and CFSE-labeled myelin debris (green) of WT and Lcn2−/− primary astrocytes with or without LPS pre-treatment (n = 5 independent primary cell cultures). Scale bar, 20 μm. B ELISA analysis for internalized MBP in primary astrocytes of two genotypes (n = 5 independent primary cell cultures; mean ± S.D.; adjusted ***P < 0.0002 vs. WT myelin; #P < 0.0083 vs. WT LPS + myelin; two-way ANOVA, repeated-measures t-test). C, D Flow cytometry showing the differences of astrocytes to internalize myelin debris (n = 5 independent primary cell cultures; mean ± S.D.; adjusted ***P < 0.0002 vs. WT myelin; #P < 0.0083 vs. WT LPS + myelin; two-way ANOVA, repeated-measures t-test). In the box plots (B, D), the middle bar represents the median, the box represents the interquartile range, and whiskers indicate the maximum and minimum values. Dots are all the data points. Source data are provided as a Source Data file.
