Fig. 6: Ablation of Lcn2 partially rescues demyelination and cognitive deficits after cortical ischemia.

A Representative colored principal eigenvector maps of DTI in WT and Lcn2^−/− mice. The numbers (B1.2, B0.6, B − 0.6 and B − 1.2) refer to the coronal slice distance (mm) anterior to the bregma (color schemes: red for left–right, blue for cranial–caudal, green for dorsal–ventral directions). B Quantification of FA value according to DTI (n = 3 mice; mean ± S.D.; adjusted **P < 0.0017 vs. WT sham; ^#P < 0.0083 vs. WT dMCAO; two-way ANOVA, repeated-measures t-test). C–E Representative black gold, LFB staining images and quantifications from dMCAO mice (n = 5 mice; mean ± S.D.; adjusted ***P < 0.0002 vs. WT sham; ^#P < 0.0083 vs. WT dMCAO; two-way ANOVA, repeated-measures t-test). Scale bar, 50 μm. F–H Representative EM images and quantifications of two genotypes (n = 5 mice; mean ± S.D.; two-way ANOVA, Tukey post hoc test or repeated-measures t-test; ***P < 0.001 vs. WT sham; ^###P < 0.001 vs. WT dMCAO for Tukey post hoc test; adjusted ***P < 0.0002 vs. WT sham; ^##P < 0.0017 vs. WT dMCAO for repeated-measures t-test). Scale bar, 2 μm. I Neurological deficit evaluation using mNSS (n = 10 mice; mean ± S.D.; adjusted **P < 0.0017, ***P < 0.0002 vs. WT sham; ^#P < 0.0083 vs. WT dMCAO; two-way ANOVA, repeated-measures t-test). J Representative swim path trace images in hidden platform test (learning) and probe trial (memory). K, L The escape latency and swim path length during learning stage (n = 10 mice; mean ± S.D.; *P < 0.05, **P < 0.01, ***P < 0.001 vs. WT sham; ^#P < 0.05, ^##P < 0.01 vs. WT dMCAO; two-way repeated-measures ANOVA, Tukey post hoc test). M, N Quadrant time and platform crossovers during probe trial (n = 10 mice; mean ± S.D.; adjusted **P < 0.0017, ***P < 0.0002 vs. WT sham; ^##P < 0.0017 vs. WT dMCAO; two-way ANOVA, repeated-measures t-test). Source data are provided as a Source Data file.