Fig. 1: Single-cell RNA-sequencing identifies the transcriptional signatures of AGM-EC that differentially support HSC generation in vitro. | Nature Communications

Fig. 1: Single-cell RNA-sequencing identifies the transcriptional signatures of AGM-EC that differentially support HSC generation in vitro.

From: Engineering a niche supporting hematopoietic stem cell development using integrated single-cell transcriptomics

Fig. 1

a Methodology for comparison of AGM-EC in supporting the generation of engrafting HSC from E9-10 AGM/P-Sp-derived VE-cadherin+ hemogenic precursors. b Donor-derived peripheral blood (PB) engraftment in recipients transplanted with the progeny of hemogenic precursors following co-culture on various independently generated AGM-EC (EC1-4). The numbers above indicate the fraction of mice with multilineage engraftment, designated by data points in red. (PB engraftment shown at ≥24 weeks post transplant, pooled from n ≥ 3 independent experiments for each AGM-EC, from embryos in 18–35 somite pair range; source data are provided as a Source Data file). c UMAP and cluster analysis of single-cell transcriptional profiles of each AGM-EC from b. (See also Supplementary Fig. 1). d Gene-set scores representing top GO terms for transcripts differentially expressed by HSC-supportive verses non-supportive AGM-EC (biological processes and molecular functions ranked by p value, see also Supplementary Data 1, Supplementary Data 2). e Gene expression heatmap for pan-endothelial marker, VE-Cadherin (Cdh5), and selected transcripts differentially expressed between clusters. (See also Supplementary Data 1).

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