Fig. 6: Functional validation of ligand–receptor interactions enables the engineering of an in vitro niche supporting HSC development.

a Methodology evaluating generation of engrafting HSC from AGM-derived V⁺61⁺E⁺ hemogenic precursors in engineered conditions. b Donor-derived peripheral blood engraftment (≥24 weeks) in recipients transplanted with the progeny of E11 AGM-derived V⁺61⁺E⁺ hemogenic precursor following co-culture in engineered conditions with immobilized Dll1-Fc, anti-Notch1/Notch2 (aN1/N2 Ab), or control (IgG). Data points in red indicate mice with multilineage engraftment (results pooled from n = 2 independent experiments). (See also Supplementary Fig. 11. Source data are provided as a Source Data file). c Donor-derived peripheral blood engraftment (≥24 weeks) in recipients transplanted with the progeny of E10 AGM-derived V⁺61⁺E⁺ hemogenic precursor following co-culture in engineered conditions as indicated (results pooled from n = 5 independent experiments). (Source data are provided as a Source Data file). d Ligand–receptor interactions recapitulated in the engineered niche. e UMAP and pseudotemporal ordering of single-cell transcriptomes of the progeny of E10 V⁺61⁺E⁺ hemogenic precursors following 6 days of culture on AGM-EC or engineered conditions. f Cell type classification based on marker genes. Undefined cell types are shown in gray. g, h Subsets of all single cells (g) and cell types (h) derived from cells cultured on AGM-EC (left panels) or engineered conditions (right panels). (See Supplementary Fig. 11).