Fig. 6: HMPS@AcDX inhibits gliosis, attenuates the activation of microglia, suppresses CSGP production, and protects axon and myelin sheath. | Nature Communications

Fig. 6: HMPS@AcDX inhibits gliosis, attenuates the activation of microglia, suppresses CSGP production, and protects axon and myelin sheath.

From: High drug-loaded microspheres enabled by controlled in-droplet precipitation promote functional recovery after spinal cord injury

Fig. 6

a Representative immunohistochemical staining of GFAP (in green) and CD68 (in red) in longitudinal sections of injured spinal cord at day-28 post injury. The nuclei of all cells were stained with DAPI (in blue). b Semi-quantification of GFAP intensity and density of microglia in the injured spinal cord. For GFAP intensity, the data are plotted as the relative ratio of the immunoreactivity near the injury site compared with that in distant area. (n = 6 per group). c Representative immunohistochemical staining of GFAP (in green) and CD68 (in red) adjacent to the lesion area. d The deposition of chondroitin sulfate proteoglycans (CSPGs) determined by CS56 antibody (in red) at day-28 after injury. e Semi-quantification of CS56 intensity increase in the traumatic lesion area. The data are plotted as the relative ratio of the immunoreactivity near the injury site compared with that in the distant area. (n = 6 per group). f Enlarged immunohistochemical staining of GFAP (in green) and CS56 (in red) adjacent to the lesion area. g Immunohistochemical staining of MBP (in green) and NF200 (in red) in injured spinal cord at day-28 post trauma. h Semi-quantification of NF200 and MBP intensity after spinal cord injury therapy. The data are plotted as the relative ratio of the immunoreactivity near the injury site compared with that in distant area. (n = 6 per group). Box plots show the minimum value, the first quartile, the median, the third quartile, and the maximum value. i Representative immunohistochemical staining of MBP (in green) and NF200 (in red) adjacent to the lesion area. The intervention groups were compared with the SCI group (*); HMPS@AcDX group was compared with the LMPS@AcDX group (#) and LAcDX group (⊥); *, ⊥ P < 0.05, **, ##, ⊥⊥ P < 0.01, ***, ### P < 0.001. Statistical significance was analyzed using one-way ANOVA followed by Fisher’s post-hoc test. Exact P values are given in the Source data file.

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