Fig. 2: SOX10 regulates the transcription of genes involved in invasion, proliferation, and cell metabolism pathways.
From: Targeting SOX10-deficient cells to reduce the dormant-invasive phenotype state in melanoma
a MeWo and A375 SOX10 knockout cell lines were generated as described in Materials and Methods. The same number of parental and SOX10 knockout cells were seeded in six-well plates for each cell line. Cells were lysed and lysates western blotted as indicated. The experiment was repeated independently three times with similar results. b A heatmap showing GSEA normalized enrichment scores (NES) for the hallmark gene sets collection for MeWo and A375 SOX10 knockout cells (guide #2 and #4) compared to parental cells. NES values are displayed for enriched gene sets, using a Benjamini-Hochberg False Discovery Rate (BHFDR) cutoff of 0.05. MeWo gRNA#2 includes combined data collected from clones # 2.1, # 2.2, and # 2.8. For all other samples (MeWo gRNA #4, and A375 gRNA#2 and gRNA#4), shown is the mean from three independent replicates generated for each clone. c Enrichment plots of EMT, Hypoxia, MYC targets-1 and MYC targets-2 comparing MeWo and A375 SOX10 knockout cells (guide #2 and #4) with parental cells. d Enrichment plots of proliferative and invasive gene signature (Verfaille, et al.) for MeWo and A375 CRISPR SOX10 knockout (guide #2 and #4) vs parental cells. BHFDR < 0.001.
