Fig. 3: SOX10 regulates the expression of genes associated with a slow-cycling and more invasive phenotype.
From: Targeting SOX10-deficient cells to reduce the dormant-invasive phenotype state in melanoma
a The same number of MeWo parental and MeWo CRISPR SOX10 knockout (clone # 2.1; 2.2; 2.8; 4.11) cells were seeded in six-well plates for each cell line. Cells were lysed and lysates western blotted as indicated. The experiment was repeated independently two times with similar results. b MeWo parental, MeWo #2.1, and MeWo #4.11 cells were plated on coverslips coated with 0.2% gelatin. The next day, cells were treated with ascorbic acid (50 µg/ml) for 6 days. Treatment was renewed every 48 h. At the end of the experiment, cells were permeabilized, fixed, and stained for FN1 and collagen IV. The experiment was performed independently twice, and representative images are shown. Scale bars, 50 μm. c Scratch-wound assay comparing MeWo CRISPR SOX10 knockout (clones # 2.1; 2.2; 2.8; 4.11) cells to parental cells. Shown is the mean ± SD from three independent experiments. p-values were calculated using a two-sided t-test and p-values for significant comparisons are shown. d Spheroid in 3D collagen comparing MeWo CRISPR SOX10 knockout (clones # 2.1; 2.2; 2.8; 4.11) cell lines to parental cells. Shown is the mean ± SD from three independent experiments. Scale bar, 25 μm. p-values were calculated using a two-sided one-sample t-test of the null hypothesis and p-values for each comparison are shown.
