Fig. 4: PRMT7 regulates monocyte recruitment.

a The number of trans-well migrating monocytes isolated from the bone marrow of WT and Prmt7^+/− mice migrating in 4 h through TNF (10 ng/ml) activated SVECs towards serum-free (SF) medium ± 100 ng/ml CCL2 (n = 3 independent experiments). b Flow cytometry plot of CCR2 expression and MFI of CCR2 in monocytes isolated from the bone marrow of WT and Prmt7^+/− mice (n = 4 per genotype). c–i Left lungs from wild-type (WT) mice were orthotopically transplanted into WT or Prmt7^+/− recipients, left for 3 weeks to recover from surgery, and then exposed to a single dose of porcine pancreatic elastase (PPE) 40 U/Kg and analyzed 28 days later (n = 4 per group). c Schematic representation of the experiment. d Representative images of immunohistochemical analysis for Galectin 3 positive macrophages (red signal, hematoxylin counterstained, scale bar 50 μm) in sections from the transplanted lungs. e Quantification of macrophage number in lung sections from (d) given as the number of positive cells per 20 random fields of view. f Bronchoalveolar lavage fluid total and macrophage cell count. g Representative images of Hematoxylin and Eosin (H&E)-stained sections from the transplanted lungs (scale bar 200 μm). h Quantification of airspace enlargement as mean chord length (MCL) in lung sections from (g). i Lung function is measured as the diffusing capacity of carbon monoxide and forced vital capacity (FVC). j–p WT and Prmt7^+/− mice were exposed to FA or cigarette smoke (CS) for 4 months (n = 4–8 per group, repeated twice). j Representative images of immunohistochemical analysis for Galectin 3 positive macrophages (red signal, hematoxylin counterstained, scale bar 100 μm) in lung sections. k Quantification of macrophage number in lung sections (n = 4 per group) from (j) given as the number of positive cells per 20 random fields of view. l Representative flow cytometry plots of whole lung single-cell suspensions to assess F4/80⁺ macrophages gated on CD45⁺ cells and CD11b vs CD11c expression gated on the F4/80⁺ cells, see Supplementary Fig. 24 for gating strategy. m Quantification of total F4/80⁺ macrophages (n = 4 for WT FA, WT CS, Prmt7^+/− FA and n = 5 for Prmt7^+/− CS) and CD11b⁺ F4/80⁺ macrophages (n = 4 for WT CS and n = 5 for Prmt7^+/− CS). n Representative images of H&E stained lung sections (scale bar 50 μm). o Quantification of airspace enlargement as mean chord length (MCL) in lung sections (n = 9 for WT FA, WT CS, Prmt7^+/− FA and n = 11 for Prmt7^+/− CS) from (n). p Lung function measured as diffusing capacity of carbon monoxide (n = 5 for WT FA, n = 6 for WT CS, n = 9 for Prmt7^+/− FA, and n = 6 for Prmt7^+/− CS). Data shown mean ± SD, P values shown in charts determined by one-way ANOVA Bonferroni’s multiple comparisons test (a, k, m, o, p), unpaired two-tailed Student’s t-test (e, f, h, i). Source data are provided as a Source Data file.