Fig. 1: TDP-43 is acetylated and its acetylation at K84 may affect its nuclear-cytoplasmic trafficking while acetylation at K136 causes a nuclear droplet-like distribution. | Nature Communications

Fig. 1: TDP-43 is acetylated and its acetylation at K84 may affect its nuclear-cytoplasmic trafficking while acetylation at K136 causes a nuclear droplet-like distribution.

From: Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43

Fig. 1

a HEK293E cells were transfected with 6xHis-tagged TDP-43, which was purified with NiNTA beads and subjected to MS. The residues marked in the domain structure of TDP-43 were found to be acetylated (black arrows). K145 has been reported to be acetylated in the literature but we could not find it in our study (grey arrow). b Immunostaining of HEK293E cells transfected with 6xHis-tagged wtTDP-43, acetyl-dead (K84R, K136R and K145R) or acetyl-mimics (K84Q, K136Q and K145Q). Scale bar represents 10 μm. c Quantification of percentage of cells with 6xHis-TDP-43 protein distribution diffuse nuclear (blue bars), nuclear clumpy (grey bars), nuclear + cytosolic, diffuse (orange bars) and nuclear + cytosolic, clumpy (yellow bars). A total of 300 transfected cells from three independent experiments per group were classified. ***p < 0.001 as measured by Chi-squared test. Source data are provided as a Source Data file.

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