Fig. 5: [K136Q]TDP-43 droplets have reduced mobility, growing in size and fusing over time. | Nature Communications

Fig. 5: [K136Q]TDP-43 droplets have reduced mobility, growing in size and fusing over time.

From: Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43

Fig. 5

a Representative images of FRAP analysis of HEK293E cells transfected with C-terminally EGFP-tagged wt and K136Q TDP-43. Two different groups of [K136Q]TDP-43-EGFP droplets based on size were determined. Time in seconds. Scale Bar represents 5 µm. b Quantification of FRAP images (three cells quantified per group). The inclusions bodies are classified as follows: diffuse TDP-43 (circles), small inclusions (triangles) and big inclusions (squares). Source data are provided as a Source Data file. c Quantification of mobile fraction of FRAP analysis of wt and K136Q TDP-43 mutants. Three cells quantified per group. Data are presented as mean values ± SEM. Unpaired, two-sided t-test was used for comparison *p < 0.05. Source data are provided as a Source Data file. d Selected frames from green fluorescence live-cell imaging of cells expressing [K136Q]TDP-43-EGFP. Close-up of granules fusing (white arrows). Time in seconds. Scale bar represents 5 µm. N = 3 biological replicates. e Histogram of K136Q TDP-43 aggregate size in HEK293E fixed 24, 48 or 72 h after transfection. A total of 100 transfected cells analyzed for each time point. Different patterns represent the respective hours after transfection. Unpaired, two-sided t-test was used for comparison. Data are presented as mean values ± SEM. **p = 0.0075. Source data are provided as a Source Data file.

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