Fig. 6: Antibodies against acetylated TDP-43 specifically recognize the modified forms. | Nature Communications

Fig. 6: Antibodies against acetylated TDP-43 specifically recognize the modified forms.

From: Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43

Fig. 6

a, b HEK293E shTDP-43 cells were co transfected with E451 plasmid and different C-terminally 6xHis-tagged TDP-43 constructs. After 24 h in the presence of 5 mM of acK cells were lysed. Samples were Western probed with antibodies against acetylated K84 (clone 9G9) and K136 (clone 14D4), respectively. N = 3 biological replicates. Source data are provided as a Source Data file. c, d HEK293E shTDP-43 cells were cotransfected with E451 plasmid and different C-terminally 6xHis-tagged TDP-43 constructs. After 24 h in the presence of 5 mM of acK cells were fixed and immunostained for 6xHis or acetylated TDP-43. Scale bar represents 10 µm. N = 3 biological replicates.

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