Fig. 7: Sirtuins 1 and 2 can deacetylate K136 of TDP-43 and prevent its aggregation. | Nature Communications

Fig. 7: Sirtuins 1 and 2 can deacetylate K136 of TDP-43 and prevent its aggregation.

From: Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43

Fig. 7

a HEK293E shTDP-43 cells were cotransfected with E451, K136TAG TDP-43 and SIRT1 or SIRT2. Twenty-four hours before lysis, 5 mM acK and DMSO, 1 µM or 10 µM Ex527 containing media was supplied to the cells. N = 3 biological replicates. Source data are provided as a Source Data file. b Stably amber suppressed shTDP-43-HEK293E cells were cotransfected with different sirtuin constructs together with K136TAG TDP-43. Cells were fixed after 24 h in the presence of 5 mM acK. Scale bar represents 10 µm. c Quantification of the number of cells with TDP-43 positive inclusions (6xHis staining). A total of 200 cells from 4 independent experiments were recorded per condition. Unpaired, two-sided t-test was used for comparison. Data are presented as mean values ± SEM. **p = 0.0083. Source data are provided as a Source Data file.

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