Fig. 2: Summary of all subcomplexes obtained in vitro and in cellula.
From: Bacterial F-type ATP synthases follow a well-choreographed assembly pathway
a List of all subcomplexes observed with LILBID-MS after in vitro assembly of isolated subunits in the presence of 2 mM ATP and 2 mM MgCl2. b SDS-PAGE (NuPAGE 4–12% Bis-TRIS) of single purified subunits α, β, γε, and δ. Affinity purification of α with Strep-Tactin- and β, γε, and δ with Ni-chelating chromatography. N = 3 independent experiments. c List of in E. coli heterologously purified F1 and subcomplexes. All complexes were analyzed with LILBID-MS and SDS-PAGE (Supplementary Fig. 7). Source data underlying Fig. 2a are provided as a Source Data file.
