Fig. 5: Suppressing chromosomal translocations down to the level of base editors by Cas9TX.
From: Cas9 exo-endonuclease eliminates chromosomal translocations during genome editing
a Editing patterns around the break site for BE4max, ABEmax, SpCas9, and Cas9TX at the RAG1C locus detected by PEM-seq. Red arrows indicate the presumed break sites. Accumulative levels of mutations, deletions, and insertions are shown at nucleotide resolution. b, c Editing efficiency (b) and the percentages of general translocations (c) for SpCas9, Cas9TX, BE4max, and ABEmax detected by PEM-seq at EMX1, C-MYC2, DNMT1-2, RAG1C, and BCL11A in HEK293T cells. One independent PEM-seq library for each locus, N = 5. Of note, the EMX1 and C-MYC2 sites were not targetable by ABEmax. Values from minimum to maximum are shown in the box. The line represents the median. d Circos plot showing the distribution of translocations for SpCas9, Cas9TX, BE4max, and ABEmax at RAG1C in HEK293T cells. Red arrows indicate the RAG1C target site. The percentages of general translocations are shown in the center.
