Fig. 3: In vitro imaging of Gram-negative and Gram-positive bacteria based on the proposed strategy.
From: Bacteria eat nanoprobes for aggregation-enhanced imaging and killing diverse microorganisms
a–d CLSM images of four different kinds of bacteria (S. aureus (SA), E. coli (EC), M. luteus (ML), P. aeruginosa (PA)) incubated with 1.0 mg mL−1 GP-AuNPs@Ce6 without (a) or with (b) 405-nm laser irradiation, or GP-dAuNPs@Ce6 without (c) or with (d) 405-nm laser irradiation. After incubation, the treated bacteria were rinsed with PBS buffer for several times. The bacterial cell concentration is ~1.0 × 107 CFU. Laser power:1.0 W cm−2, irradiation time: 25 min. Scale bars, 10 μm. e Confocal fluorescence images of pure human blood, the mixture of human blood and EC or SA after incubation with GP-dAuNPs@Ce6. Arrows indicate red blood cells (RBCs). Scale bars: 25 μm. All imaging experiments were repeated three times with similar results.
