Fig. 3: Characterization of multiple-substitution hMPV F variants.
From: Structure-based design of prefusion-stabilized human metapneumovirus fusion proteins
a–c SEC of purified multiple-substitution hMPV F variants. A vertical dotted line indicates the peak retention volume for the hMPV F base construct. d DSF analysis of thermostability of multiple-substitution F variants. The vertical dotted line indicates the melting temperature for the base construct. e SEC trace of DS-CavEs2 purified from a 1 L culture of FreeStyle 293-F cells. f Binding of heat-treated DS-CavEs2 or DS-CavEs2 stored for 2.5 months at 4 °C to MPE8 Fab measured by biolayer interferometry. A vertical dotted line indicates the end of the association step. Untreated DS-CavEs2 was included as a control. Source data are provided as a Source Data file for panels a–f.
