Fig. 1: Gpr125 is expressed at predicted sites of stem/progenitor activity during pubertal mammary development.
From: Gpr125 is a unifying hallmark of multiple mammary progenitors coupled to tumor latency
a Left: Schematic of Gpr125 protein, Right: Gpr125-β-gal fusion protein. N-terminus (N), leucine-rich repeats (LRR), immunoglobulin-like domain (Ig), hormone-binding domain (HBD), GPCR autoproteolytic-inducing (GAIN) domain, transmembrane region (TM), and cytoplasmic region (C). b–f Gpr125-β-gal expression in mammary whole mounts from pre-pubertal (3w n = 5) and pubertal (4w n = 4) (5w n = 2) and (6w n = 11) nulliparous mice. Scale bar = 2 mm. g Section of X-gal (blue) stained mammary whole-mount counterstained with nuclear fast red (NFR), shows Gpr125 expression in the cap cell layer of terminal end buds (TEB) and in cells dispersed along the basal layer of subtending ducts (arrows) n = 4. The inset box is a higher magnification of the area indicated by arrows. h–m X-gal (blue) stained sections of TEB with immunolocalization (brown stain) of (h–j) basal markers: smooth muscle actin (SMA), p63, Keratin (K14); Note the occasional cells expressing Gpr125 within the body layer all express basal cap cell markers; k–m luminal markers: E-cadherin (Ecad), estrogen receptor (ER), progesterone receptor (PR); n, o markers of proliferative status: (n) proliferating nuclear cell antigen (PCNA) and (o) p27. Scale bar = 50 µm. n = 3 mice (h–k, n = 3, l, m, o, n = 2 mice/antigen).
