Fig. 6: Gpr125 + progenitors are expanded in MMTV-Wnt1 tumors and retain embryonic features and bipotency.
From: Gpr125 is a unifying hallmark of multiple mammary progenitors coupled to tumor latency
a–c X-gal (blue) stained mammary whole mounts from 8-week-old MMTV-Wnt1;Adgra3lz/+ mice (n = 23 mice) show robust Gpr125-β-gal expression in hyperbranched ductal tips (c) and nipple-proximal zones compared to (b) control Adgra3lz/+ littermate. d X-gal (blue) section of hyperbranched ductal tip counterstained with NFR presenting basal restriction of Gpr125-β-gal expression in 12-week-old MMTV-Wnt1;Adgra3lz/+ mice. e-h X-gal (blue) stained whole mount and sections counterstained with NFR of MMTV-Wnt1;Adgra3lz/+ tumor (g–k) showing Gpr125 cells devoid of immunolocalization (brown) for SMA, K14 or K8, but expressing p63 and Tcf1 (n = 4 tumors/marker). l Tcf1 expression (red) in the cap cells of normal TEB (n = 3 mice). m–o Lineage tracing strategy in 5-week-old MMTV-Wnt1;Adgra3lz/+ produced both (n) K5 + (green) and (o) Ecad + ;tdT + (green/red) cells in hyperplastic glands at 12 weeks. DAPI = blue nuclear staining. One gland from each of the five mice were analyzed.
