Fig. 7: High Gpr125 expression is predictive of poor outcome.
From: Gpr125 is a unifying hallmark of multiple mammary progenitors coupled to tumor latency
a X-gal/NFR stained sections showing higher Gpr125-β-gal expression in a short (7w) latency MMTV-Wnt1;Adgra3lz/+ tumor (Wnt1-early) versus a long (36w) latency (Wnt1-late) tumor. Scale bar 100 µm and 50 µm. (n = 5 tumors/subtype). b qRT-PCR analysis of relative Adgra3 mRNA expression in early and long latency tumors MMTV-Wnt1;Adgra3lz/+ tumors (n = 3 tumors/subtype, **p = 0.0052). c Representative FACS dot plots of total MECs isolated from MMTV-Wnt1;Adgra3lz/+ hyperplastic uninvolved glands and associated short and long latency tumors stained with CD24 and CD49f. FDG+ cells in: 36w tumor = 13331; 36w hyperplasia = 4048; 7w tumor = 64889; 7w hyperplasia = 13156. Plots are representative of three independent experiments. d ADGRA3 expression in (left) ER- (n = 445) and ER+ (n = 1459) and in (right) PR- (n-895) and PR+ (n = 1009) BC patient samples from the METABRIC dataset **p = 0.0042, ****p < 0.0001. e, f ADGRA3 expression in BC patient samples from TGCA PanCancer Atlas (n = 1084) (e) and METABRIC (n = 2509) (f) datasets classified using the PAM50 geneset; ****p < 0.0001. g Integrative clusters within the METABRIC dataset showing high ADGRA3 expression levels in cluster 10; ****p < 0.0001, Mann–Whitney test two tailed. h ADGRA3 expression in human breast cancer cell line from Depmap database (https://depmap.org/portal/) **p = 0.0068, ***p = 0.0002, Unpaired t test two-tailed. i–k Kaplan–Meyer curves depicting ‘Relapse-free survival’ and ‘Distant Metastasis-Free Survival’ in months for high (red lines) and low (black lines) ADGRA3 mRNA expressing human basal-type (h, i) and basal-like-1 TNBC (j) breast cancer subtypes. Auto best fit cutoff was used to divide patients into high and low expression (indicated in beehive plots) sourced from (https://kmplot.com/analysis/index.php?p=service&cancer=breast) KMplotter75. logrank p-value and Hazard Ratio (HR) indicate a significant association between high expression and poor prognosis. Each scatter dot plot represents the mean ± SEM. Source data for this figure are provided as a Source Data file.
