Fig. 6: CG and non-CG methylation jointly regulate the activity of RAM.

a Quantification of root length of 9-day-old WT, ddcc, met1-9, and mddcc seedlings. The data are the means ± SD of the biological repeats. b Representative images of EdU labeling in the RAM of roots of 11-day-old WT, ddcc, met1-9, and mddcc seedlings. Images framed with a red dotted line show a higher exposure version of the images on the left. Scale bar: 50 μm. Experiments were independently repeated two times with similar results. c Distance from the QC to xylem lignification in roots of 9-day-old WT, ddcc, met1-9, and mddcc mutants. The data are the means ± SD of the biological repeats. d Cleared roots stained with calcofluor white (blue) and basic fuchsin (red). White arrowheads indicate the site of appearance of the first protoxylem cells; red arrowheads indicate root hairs. Scale bar: 100 μm. Experiments were independently repeated two times with similar results. e Representative images of root meristems from 9-day-old WT, ddcc, met1-9, and mddcc seedlings. Cell outlines were visualized with calcofluor white. Blue and white arrowheads indicate the QC and the cortex transition boundary, respectively. Scale bars: 100 μm. Experiments were independently repeated two times with similar results. Source data are provided as a Source Data file.