Fig. 4: Impact of nuclear actin upon the organisation of RNAPII.
From: Myosin VI regulates the spatial organisation of mammalian transcription initiation
A Example STORM render image of myosin VI and RNAPII-pSer5 following treatment with Latrunculin B (LatB), as described in the methods (scale bar 2 μm). Further widefield example images are shown in Supplementary Fig. 13D. B (left) Example widefield image of YFP-NLS-R62D actin following transfection. (right) Example STORM render image of RNAPII-pSer5 following transfection of YFP-NLS-R62D actin (scale bar 2 μm) WT YFP-NLS actin control is Supplementary Fig. 13E. C Cluster analysis of RNAPII-pSer5 nuclear organisation following treatment with LatB (n = 12) or transfection with YFP-NLS-R62D Actin (n = 11). D Cluster analysis of myosin VI nuclear organisation following treatment with LatB (n = 12). Individual data points correspond to the average value for a cell ROI. The values represent the mean from the ROIs for each condition (Only statistically significant changes are highlighted **p < 0.01; ***p < 0.001; ****p < 0.0001 by two-tailed t test compared to normal conditions).
