Fig. 6: Perturbation of myosin VI impacts chromatin organisation and cell growth.
From: Myosin VI regulates the spatial organisation of mammalian transcription initiation
A Example immunofluorescence staining against stated histone marks (magenta) and DNA (blue) in HeLa cells under normal and TIP-treated conditions (scale bar 10 μm). B Chromatin accessibility assay. qPCR was performed against the stated genes on chromatin purified from HeLa cells in the presence and absence of TIP. Chromatin was divided in to nuclease and control samples where nuclease activity was determined by difference between the two samples (Fold enrichment). HBB represents a repressed, inaccessible, control gene. Error bars represent SEM from three independent experiments. C Real-time growth of HeLa cells (red) and corresponding measurements following MVI (MVI) siRNA knockdown (green) and mock transfection control (blue). Data represent three independent measurements and error bars show SEM. Example images at start and 60 h time points are shown in (D) (scale bar 300 μm in all images). Western blots are shown in Supplementary Fig. 17 for the MVI knockdown.
