Fig. 1: Experimental setup for capturing variation in MIRS caused by the laboratory of origin, individual genetic differences and natural environment.
From: Rapid age-grading and species identification of natural mosquitoes for malaria surveillance

To disentangle genetic and environmental effects, mosquitoes were obtained from either laboratory-bred colonies or from genetically heterogeneous wild larvae; half of the laboratory larvae were then reared and allowed to develop through the adult stage in semi-field conditions, which offer ecologically realistic conditions while still allowing control of mosquito age.