Fig. 2: NaX pore module structure reveals a nonconductive state.
From: Structure-guided unlocking of NaX reveals a non-selective tetrodotoxin-sensitive cation channel
a NaX pore volume shown as gray surface with DII and DIV in cartoon rendering (DI and DIII omitted for clarity). b View of the S6-helices with side-chains lining the activation gate shown. Orthogonal view provides a wider perspective with DIII and DIV colored orange and pink, and the IFI-motif (green) from the DIII–DIV linker shown in stick and semi-transparent surface representation. c Orthogonal views sliced through the pore module highlighting lateral fenestrations and bound lipids. The phosphatidylethanolamine that crosses the S6-gate is in purple stick representation. d Similar to middle panel c, but with cryo-EM map shown in blue mesh representation. e Location of S6-gate hydrophobic side-chains targeted by pore-wetting mutations. f Representative currents from Xenopus laevis oocytes expressing the NaX-QTT construct with voltage protocol indicated. g Data summary of independent experiments with indicated constructs (see Supplementary Fig. 8). Data are shown as mean ± SD; ns not significant; ****p < 0.0001; one-way ANOVA with Dunnett’s test (against NaX-QTT + H2O). Exact p-values and statistical parameters are provided in Source Data. Numbers of biological replicates (n) are indicated.
