Fig. 2: Training on cleavage and binding for moderately mismatched targets. | Nature Communications

Fig. 2: Training on cleavage and binding for moderately mismatched targets.

From: A kinetic model predicts SpCas9 activity, improves off-target classification, and reveals the physical basis of targeting fidelity

Fig. 2

a Training data (triangles) for effective cleavage rates (NucleaSeq) on single-mismatch targets, and the model estimates (line). b Training data (upper-left triangle) for effective cleavage rate on double-mismatch targets, and the model estimates (lower-right triangle). c Correlation plot for all effective cleavage rate data used for training (single- and double-mismatch targets). d Training data (triangles) for effective association constant (CHAMP) on single-mismatch targets, and the model estimates (line). e Training data (upper-left triangle) for effective association constant on double-mismatch targets, and the model estimates (lower-right triangle). f Correlation plot for all effective association constant data used for training (single- and double-mismatch targets). All data is averaged over mismatch type (see Supplementary Data 1). The quoted correlation coefficients are Pearson-correlation coefficients, and correlation plots are displayed with log-scales to show the quantitative agreement also for weak targets. The dashed line in the correlation plots correspond to perfect quantitative prediction.

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