Fig. 2: Prey attachment and entry times of B. bacteriovorus wild-type and ∆bd1075.
From: Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria
a Schematic to illustrate the measurement of attachment and entry times. Attachment time: number of frames (1 frame = 1 min) between initial predator attachment to prey and the first sign of predator entry into prey (stages 1–2). Entry time: number of frames between the first sign of predator entry and the predator residing completely inside the prey bdelloplast (stages 2–4). b Duration of attachment to and c entry into E. coli S17-1 prey by B. bacteriovorus HD100 wild-type (WT), ∆bd1075, and ∆bd1075 complemented by single-crossover reintroduction of the bd1075HD100 gene into the genome of the mutant: ∆bd1075 (comp), measured by time-lapse microscopy. n = 90 cells, with 30 cells analyzed from each of 3 biological repeats. Box: 25th to 75th percentiles; whiskers: range min-max; box line: median; ns: non-significant (p > 0.05); ****p < 0.0001; Kruskal–Wallis test with Dunn’s multiple comparisons. Additional data plots are included in Supplementary Fig. 8, Supplementary Fig. 9, and Supplementary Fig. 10. Examples of B. bacteriovorus invasions are shown in Supplementary Fig. 11 and Supplementary Movies 1 (WT), 2 (∆bd1075) and 3 (∆bd1075 (comp)). Source Data are provided as a Source Data file.
