Table 1 Quantification of muropeptides released from B. bacteriovorus HD100 sacculi.

From: Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria

Muropeptide

Relative peak area (%)a in B. bacteriovorus strain

WT HD100

Δbd1075

Δbd1075 (pbd1075HD100)

Δbd1075 (pEV)

Monomers

 Tri

14.4 ± 1.0

n.d.*b

14.8 ± 1.2

n.d.*

 Tetra

9.6 ± 0.8

23.7 ± 0.8*

0.6 ± 0.9*

23.1 ± 2.7*

 Di

2.1 ± 0.1

n.d.*

7.2 ± 0.5*

n.d.*

 Penta

12.8 ± 2.5

11.9 ± 3.6

12.2 ± 4.2

14.8 ± 0.0

 Monomer anhydroMurNAc

2.2 ± 3.0

2.3 ± 3.2

2.6 ± 3.7

2.5 ± 3.6

 Monomers (Total)

38.9 ± 0.5

35.6 ± 4.3

34.9 ± 2.6

37.8 ± 2.6

Dimers

 TetraTri

14.9 ± 0.7

0.5 ± 0.7*

26.4 ± 1.5*

n.d.*

 TetraTetra

18.6 ± 0.6

33.2 ± 0.7*

7.8 ± 1.4*

29.6 ± 4.7*

 TetraPenta

12.2 ± 1.0

12.5 ± 0.3

14.7 ± 2.9

13.1 ± 0.2

 Dimer anhydroMurNAc

17.3 ± 0.8

16.4 ± 0.6

20.5 ± 4.4

17.0 ± 0.3

 Dimers (total)

45.6 ± 2.3

46.1 ± 0.6

48.9 ± 2.9

42.7 ± 4.5

Trimers

 TetraTetraTri

1.6 ± 0.4

n.d.*

3.8 ± 0.2*

n.d.*

 TetraTetraTetra

6.9 ± 0.5

11.2 ± 0.8*

4.9 ± 1.0

10.6 ± 2.2*

 TetraTetraPenta

6.9 ± 3.6

7.1 ± 3.2

7.4 ± 4.3

8.8 ± 4.0

 Trimer anhydroMurNAc

7.0 ± 0.3

9.5 ± 1.0*

6.2 ± 1.3

10.3 ± 1.0*

 Trimers (total)

15.4 ± 2.8

18.3 ± 4.0

16.2 ± 5.5

19.4 ± 1.8

Dipeptides (total)

2.1 ± 0.1

n.d.*

7.2 ± 0.5*

n.d.*

Tripeptides (total)

22.4 ± 0.6

0.2 ± 0.3*

29.3 ± 0.5*

n.d.*

Tetrapeptides (total)

54.3 ± 1.1

79.3 ± 2.0*

41.4 ± 4.2

75.7 ± 1.4*

Pentapeptides (total)

19.0 ± 1.3

18.2 ± 0.9

19.5 ± 0.6

21.8 ± 5.0

AnhydroMurNAc (total)

13.1 ± 3.3

13.6 ± 2.6

14.9 ± 5.4

14.5 ± 3.8

Average chain length

7.9 ± 2.0

7.5 ± 1.4

7.2 ± 2.6

7.1 ± 1.9

Degree of crosslinkage

33.1 ± 0.7

35.3 ± 2.8

35.3 ± 2.2

34.3 ± 1.0

% peptides in crosslinks

61.1 ± 0.5

64.4 ± 4.3

65.1 ± 2.6

62.2 ± 2.6

  1. Values represent the relative percentage area of each muropeptide peak in Fig. 4. Numbers with an asterisk differ from WT HD100 by more than 30% and values that are additionally emboldened differ by more than 50%. Source Data are provided as a Source Data file.
  2. aValues are mean ± variation of two biological replicates.
  3. bn.d. not detected.
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