Fig. 7: β-CATENIN regulates the transcription of DKK1.
From: Cancer stem cell regulated phenotypic plasticity protects metastasized cancer cells from ferroptosis
a Immunoblot assessment of the protein levels of β-CATENIN in the matched mammosphere-enriched BCSCs and parental MCF-7 or T47D cells. b Immunoblot assessment of the protein levels of β-CATENIN and DKK1 in MCF-7 or T47D cells transfected with two different β-CATENIN shRNAs. c Immunoblot assessment of the protein levels of β-CATENIN and DKK1 in MCF-7 cells treated with CHIR99021 or XAV939. d Cell viability of MCF-7 cells cultured with the CM derived from parental cells or mammosphere-enriched shCONT BCSCs or shCTNNB1-BCSCs and treated with a graded concentration of Erastin for 48 h. e Schematic representation of the predicted TCF4 binding sites in the DNA promoter region of DKK1 based on rVista 2.0 software. f ChIP-sequencing data shows the enrichments of TCF4 around the promoter region of DKK1 in MCF-7, HCT116, and Panc1 cells (GSE31477). g ChIP-sequencing data shows the enrichments of β-CATENIN around the promoter region of DKK1 in cells treated with or without Wnt3a (GSE64758). h Regulation of wild-type or mutant (TCF4 binding site deleted) DKK1 promoter activities by β-CATENIN were determined by luciferase reporter assay. Renilla luciferase activity as input control. i Binding of TCF4 to the DKK1 promoter region in MCF-7 cells was examined by ChIP assay. j The binding of β-CATENIN to the DKK1 promoter region in BCSCs or parental MCF-7 cells was examined by ChIP assay. Results are shown as mean ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant (One-way ANOVA followed by Tukey’s multiple comparison test in (h), others two-way ANOVA test). Source data are provided as a Source Data file.
