Fig. 4: Reduced oligodendrocyte differentiation and myelin gene expression at the onset of myelination in the mutant mPFC.

a Experimental plan. b Immunostaining of OPCs and oligodendrocytes (OLs) for PDGFRα (Pα, green), Olig2 (white) and CC1 (magenta) in p14 medial prefrontal cortex (mPFC). c Density of OPCs and OLs in ctl and cKO mPFC at p10 and p14. (OPC: p10: ctl =4  mice, cKO = 4 mice; p14: ctl = 10 mice, cKO = 9 mice; OL: p10: ctl = 5 mice, cKO = 4 mice; p14: ctl = 5 mice, cKO = 4 mice, two-sided unpaired t-tests). d Percentage of OLs of the complete lineage at p14 (ctl = 5 mice, cKO = 4 mice, two-sided unpaired t-tests). e Quantitative analysis of MBP mRNA level in ctl and cKO mPFC at p14. (ctl = 4 mice, cKO = 4 mice, two-sided unpaired t-test). Immunostaining (f, g) and quantification (h) of OPCs (Pα⁺, green in f) and OLs (CC1⁺, green in g) incorporated with BrdU (magenta). (OPC: ctl = 5 mice, cKO = 5 mice, two-sided unpaired t-test; OL: ctl = 4 mice, cKO = 5 mice, two-sided unpaired t-test). i Experimental design of j–l. j Immunostaining of OLs (CC1⁺, green) combined with tdTomato (tdT, magenta) expression in the mPFC at 2w. k Recombination efficiency of OLs. (2w: ctl = 3 mice, cKO = 4 mice, two-sided unpaired t-test; 9w: ctl = 4 mice, cKO = 5 mice, two-sided unpaired t-test). l Comparison of recombined and non-recombined OL densities (with or without tdT expression). (2w: ctl = 3 mice, cKO = 4 mice, two-sided unpaired t-tests; 9w: ctl = 4 mice, cKO = 5 mice, two-sided unpaired t-tests). Data are shown as mean ± SEM in c–e, h, k and l. Source data are provided as a Source Data file.