Fig. 2: Gcn5-containing complex is a histone benzoyltransferase.
From: Global profiling of regulatory elements in the histone benzoylation pathway
a Fluorescence quantitative western blot analysis comparing the Kbz and Kac levels in WT (BY4742) and HAT-deletion strains. Total H4 was used as the loading control. The fluorescence signals from mutant strains were normalized by setting the WT fluorescence signal to 1.0 and were labeled in the image. Source data for panels (a–c) are provided in the Source Data file. b The Ada2-Gcn5 subcomplex could catalyze acetylation of the histone octamer in vitro. The reaction products were detected by western blot analysis using a pan-Kac antibody. c Histone benzoylation can occur by enzymatic and non-enzymatic mechanisms. The reaction products in different combinations were detected by western blot analysis using a pan-Kbz antibody. d The list of LC-MS/MS-identified histone benzoylation sites deposited by the non-enzymatic mechanism and by Gcn5-Ada2. The unique sites catalyzed by Gcn5-Ada2 are labeled red. e Gcn5-Ada2 catalyzed histone benzoylation more efficiently than spontaneous reaction. The Y-axis represents the ratio of MS/MS peak areas under enzymatic and non-enzymatic conditions. This result is from a single experiment.
