Fig. 7: Proteome-wide screening of Kbz-modified proteins in yeast cells.
From: Global profiling of regulatory elements in the histone benzoylation pathway
a The workflow used for HPLC–MS/MS-based non-histone Kbz sites identification in S. cerevisiae. b The MS/MS spectrum of an Eno1 peptide (LAK+104.0268LNQLLR) harboring one benzoylated site. Predicted y- and b-type ions are listed above and below the peptide sequence, respectively. Matched ions are labeled in the spectra. c Distribution of non-histone Kbz sites based on the site number per protein. d Venn diagram showing the subcellular compartment distribution of non-histone Kbz proteins. e Gene ontology analysis associated with significant regulated genes (P < 0.05) of non-histone Kbz proteins. P values are derived from one-sided Fisher’s exact test. f Overview of glycolysis pathway. The enzymes with identified benzoylation sites are shown in red. g Eight benzoylation sites on Eno1 are shown in the structural model of Eno1 (PDB: 1ONE). The Eno1 substrate, 2-phosphoglycerate, is shown in cyan. K346, K397, and K409 are adjacent to the substrate-binding pocket.
