Fig. 2: PDCoV binds to sites on APN that are different from alphacoronaviruses HCoV-229E and PRCoV.
From: Structures of a deltacoronavirus spike protein bound to porcine and human receptors
a (Left panel) Overlay of PDCoV and PRCoV footprints on pAPN. pAPN is shown as surface representation, with DI–DIV colored in orange, light green, pink, and cyan respectively. Residues on pAPN contacting PDCoV RBD and PRCoV RBD are colored in blue and magenta, respectively. The boundaries of PDCoV and PRCoV footprints are circled with red dotted lines. (Middle panel) PDCoV RBD footprint on pAPN. (Right panel) PRCoV footprint on pAPN. PDCoV RBD footprint engages both DII and DIV of pAPN while PRCoV RBD largely targets DIV of pAPN. b (Left panel) Overlay of PDCoV and HCoV-229E footprints on hAPN. hAPN is depicted as surface representation. DI–DIV of hAPN are colored in orange, dark green, light blue, and brown, respectively. Residues on hAPN that bind to PDCoV RBD and HCoV-229E RBD are colored as in (a). The boundaries of PDCoV RBD and HCoV-229E RBD binding sites are circled with red dotted lines. (Middle panel) PDCoV RBD footprint on hAPN. (Right panel) HCoV-229E footprint on hAPN. PDCoV RBD binding sites on hAPN are also located both on DII and DIV of hAPN, whereas HCoV-229E RBD mainly binds to DII of APN. c Sequence alignment of pAPN and hAPN. Residues on pAPN/hAPN binding to PDCoV RBD, PRCoV RBD, and HCoV-229E RBD are marked according to the code of the key above the sequences.
