Fig. 2: Unique binding mode of galanin at GAL1R and GAL2R.
From: Molecular basis for allosteric agonism and G protein subtype selectivity of galanin receptors
a–o The binding modes of peptides at different peptide GPCRs. The peptides are shown in cartoon presentation and colored as indicated. The peptide-binding pockets are displayed as transparent surfaces with the same colors of specific peptides. The volume of each orthosteric pocket was measured by POVME 2.049. The N-terminus (N-Term), C-terminus (C-Term) of peptides and volumes of peptide-binding pockets are indicated. The depths of galanin-binding and the pocket in galanin receptors are highlighted as dashed reference lines in red and black, respectively. a Galanin in GAL1R; b galanin in GAL2R; Tilt angles (20°) compared to cell membrane and Y9P of galanin are indicated. Extra binding pockets beneath galanin are highlighted as red dashed ovals. c DAMGO in mu-opioid receptor (μOR, PDB: 6DDE); d Ghrelin in ghrelin receptor (GHSR, PDB: 7F9Y); e WKYMVm in formyl peptide receptor 2 (FPR2, PDB: 6OMM); f Neurotensin 8-13 (NTS8-13) in neurotensin receptor 1 (NTSR1, PDB: 6OS9); Angiotensin II (Ang II) in angiotensin II receptor type 1 (AT1R, PDB: 6OS0, g) and type II (AT2R, PDB: 6JOD, h); i Des-Arg10-kallidin in bradykinin B1 receptor (B1R, PDB: 7EIB); j Bradykinin in bradykinin B2 receptor (B2R, PDB: 7F2O); k CCK-8 in cholecystokinin A receptor (CCKAR, PDB: 7EZM); l Neuromedin U (NMU) in neuromedin U receptor 1 (NMUR1, PDB: 7VG6); m Arginine vasopressin (AVP) in vasopressin receptor 2 (V2R, PDB: 7DW9); n, o α-melanocyte-stimulating hormone (α-MSH) in melanocortin 1 receptor (MC1R, PDB: 7F4D, n) and melanocortin 4 receptor (MC4R, PDB: 7F53, o).
