Fig. 7: Role of ICL2 of GAL2R in Gq-coupling selectivity.
From: Molecular basis for allosteric agonism and G protein subtype selectivity of galanin receptors
a, b The effects of ICL2 swapping between GAL1R (139-144) and GAL2R (129-135) on Gq-mediated signaling (calcium signal). GAL1R (GAL2R-ICL2m) represents mutated GAL1R containing the ICL2 from GAL2R. c, d The effects of ICL2 swapping between GAL1R (139-144) and GAL2R (129-135) on Gi-mediated signaling (cAMP signal). The effects of GAL2R ICL2 (129-135) substitution of Gs-coupled β2AR (137-142, e) and D1R (127-132, f) on Gq-mediated signaling. The effects of GAL2R ICL2 (129-135) substitution of Gi-coupled 5-HT1AR (140-145, g) and μOR (173-178, h) on Gq-mediated signaling. Models to demonstrate roles of ICL2 on enhanced Gq-coupling activity of Gs- (i) and Gi-coupled class A GPCRs (j). Data shown are mean ± S.E.M. of three independent experiments (n = 3). Source data are provided as a Source Data file.
