Table 1 Semen analysis using CASA in the mouse model of Cep128.
From: CEP128 is involved in spermatogenesis in humans and mice
Adult male mice | |||
|---|---|---|---|
WT | KO | KI | |
Semen parameters | |||
Sperm concentration (106/ml)a | 27.80 ± 5.54 | 15.32 ± 1.43 | 11.28 ± 1.87 |
Motility (%) | 56.60 ± 8.82 | 3.09 ± 0.90 | 2.87 ± 1.67 |
Progressive motility (%) | 54.66 ± 11.30 | 1.82 ± 0.84 | 1.75 ± 1.11 |
Sperm locomotion parameters | |||
Curvilinear velocity (VCL) (μm/s) | 57.05 ± 7.20 | 10.16 ± 1.39 | 8.11 ± 2.28 |
Straight-line velocity (VSL) (μm/s) | 17.37 ± 3.79 | 0.92 ± 0.30 | 0.34 ± 0.21 |
Average path velocity (VAP) (μm/s) | 26.87 ± 2.56 | 3.00 ± 1.50 | 1.81 ± 0.82 |
Amplitude of lateral head displacement (ALH) (μm) | 0.98 ± 0.16 | 0.21 ± 0.02 | 0.17 ± 0.04 |
Linearity (LIN) | 0.37 ± 0.05 | 0.05 ± 0.02 | 0.04 ± 0.04 |
Wobble (WOB = VAP/VCL) | 0.48 ± 0.10 | 0.29 ± 0.09 | 0.22 ± 0.05 |
Straightness (STR = VSL/VAP) | 0.64 ± 0.09 | 0.33 ± 0.09 | 0.18 ± 0.06 |
Beat-cross frequency (BCF) (Hz) | 6.29 ± 0.67 | 0.81 ± 0.33 | 0.35 ± 0.20 |
