Fig. 3: CRISPR-Kill-mediated floral tissue elimination by cleaving 45S rDNA repeats in Arabidopsis.
From: Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
a The floral homeotic protein, APETALA1 (AP1), is highly expressed in the early floral meristem at flower development stages 1 and 2 (red). At development stage 3, the expression is restricted to specific tissue, which is determined to form sepals and petals. b Schematic representation of the ap1 mutant phenotype: a disturbed flower architecture with an extra set of carpel and stamen instead of sepals and petals. c A comparison of survival rates between the T1 control line (pAP1-SaCas9-ADH1) and the T1 flower-specific CRISPR-Kill line (pAP1-SaCas9-ITS2-1) revealed no significant differences (n = 3 biologically independent experiments). Data are presented as normalized mean values ± SD. Statistical differences were calculated using the two-tailed t-test with unequal variances. d Representative floral phenotypes of pAP1-SaCas9-ADH1 control line and pAP1-SaCas9-ITS2-1 CRISPR-Kill line. Bar = 1 mm. Statistical differences were calculated using the two-tailed t-test with unequal variances. Source data are provided as a Source data file.
