Fig. 4: Deep sequencing of floral CRISPR-Kill lines.
From: Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
The target site of ITS2-1 was analyzed via deep sequencing in floral tissue of two ADH1 control lines (#81 and #82) and ten ITS2-1 lines. The CRISPR-Kill lines were subdivided according to phenotype in ‘no phenotype’ (#5 and #3), ‘intermediate phenotype’ (#1, #4, and #2), and ‘strong phenotype’ (#26 to #30). The reads of the two ADH1 lines showed no repair events (gray), only also naturally occurring 1 bp-deletions (blue). In contrast, analysis of the CRISPR-Kill lines with a distinguishable phenotype revealed repair events like deletions (blue), insertions (red), and InDels (yellow), at the break site, with mutation frequencies linked to the severity of the phenotype. Source data have been deposited in SRA [accession: PRJNA726366].
