Fig. 5: Establishment of a centromere-specific CRISPR-Kill system in Arabidopsis.
From: Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
a The core centromere is characterized by a highly repetitive cluster of functional tandem repeats. The yellow triangle represents the cleavage site for SaCas9 within the centromere repeats (SaCas9-Cen). b Transformation of Col-0 plants with Ubi-SaCas9-Cen resulted in a reduction of the survival rate of T1 plants by 80% (n = 3 biologically independent experiments). Data are presented as normalized mean values ± SD. P = 0.00064. c Floral-specific expression of SaCas9-Cen using the AP1-promoter did not result in a reduction of the survival rate (n = 3 biologically independent experiments). Data are presented as normalized mean values ± SD. The phenotypical analysis revealed an ap1-mutant phenotype with disturbed floral architecture in most cases. Bar = 1 mm. Statistical differences were calculated using the two-tailed t-test with unequal variances: ***P < 0.001. Source data are provided as a Source data file.
