Fig. 7: CRISPR-Kill-mediated lateral root elimination in T2 lines by cleavage of 45S rDNA.
From: Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats
a Total number of lateral roots in control line and rDNA CRISPR-Kill lines. The control line, pXPP-SaCas9-ADH1, showed an average of 11 lateral roots per plant, whereas the ITS2-1-CRISPR-Kill line showed a 3-fold decrease and only an average of 3 lateral roots per plant. P < 0.0001. b Lateral root length in relation to main root length. A 3-fold decrease could also be observed in the relative lateral root length of the ITS2-1-CRISPR-Kill line. While the sum of the length of all lateral roots in the control line was on average 3.6 times longer than the main root, the sum of the length of all lateral roots in the ITS2-1 CRISPR-Kill line was on average only 1.1 times longer than the main root. P < 0.0001. Data is presented as box plots (n = 30), boxes show the first to third quartile with median, whiskers encompass 1.5x the interquartile range reaching to minimum and maximum, and data beyond that threshold is indicated as outliers. P values were calculated using the one-way ANOVA-test: ****P < 0.0001. Source data are provided as a Source data file.
