Fig. 4: RG4 in murine Tmprss2 mRNA inhibits its protein expression.
From: RNA G-quadruplex in TMPRSS2 reduces SARS-CoV-2 infection
a RG4 potential of mouse Tmprss2 mRNA was predicted by QGRS-Mapper. b CD spectrum (left panel), NMM (middle panel), and ThT (right panel) fluorescence emission spectra of PQS-1017, PQS-1370, and PQS-1585 RNA under KCl or LiCl conditions. c CD-melting measurements of PQS-1370-WT RNA under KCl or LiCl conditions. d Levels of endogenous TMPRSS2 protein in LLC cells treated with PDS (10 μM), cPDS (10 μM), or TMPyP4 (10 μM). e The full-length ORF region of murine Tmprss2 or its RG4-mutant sequence was inserted into the MCS of pcDNA3.1 vector. The bold letters represent the G-tracts and the underlined letters represent mutations. f, g Levels of HA-TMPRSS2 protein in LLC (f) and H1299 (g) cells transfected with mG4WT or mG4mut plasmids. h, i Levels of HA-TMPRSS2 protein in LLC (h) and H1299 (i) cells transfected with mG4WT (left panel) or mG4mut (right panel) plasmids, and stimulated with PDS (10 μM), cPDS (10 μM), or TMPyP4 (10 μM). Source data are provided as a source data file.
